Chairs: Dr Fernando Larcher (CIEMAT-UC3M), Dr Diego Velasco (UC3M)
To be eligible to a course, you should be an active ESDR member OR an EADV Resident and you should fully complete the application procedure.
Application deadline 2024.03.31.
GENERAL INFORMATION
The number of available course places per year is 20.
The selection of candidates is made based on CVs, year of residency and the number of courses previously attended. Complete the document “EADVESDR Summer Research Workshop 2024″ and send it to Office@esdr.org
EADV/ESDR Resident courses are for dermatology and venereology residents (either EADV members or ESDR members).
EADV/ESDR also offers accommodation, catering, and a social event during the course.
Preliminary program – as of 7.02.2024
Course Description
This workshop offers a comprehensive examination of experimental skin regenerative medicine, emphasizing on cell and gene-based approaches. Participants will gain proficiency in culture and manipulation of skin cells in both 2D and 3D modalities, focusing on preserving stem cell functionality and regenerative capacities. The course addresses the application of these strategies in scenarios such as chronic wounds, large skin losses from trauma, and genetic disorders. Essential wound healing concepts are covered, enabling participants to tackle specific challenges with appropriate therapeutic tools.
The curriculum also delves into advanced skin gene therapy strategies (e.g. genome editing) to address inherited skin defects impairing regeneration. Discussions extend to pre-clinical models and clinical applications, providing a comprehensive understanding of translational aspects in regenerative medicine.
Additionally, the course explores the complexity of cellular populations influencing skin physiology. Participants will learn to analyze and manage large datasets derived from regenerative skin approaches, incorporating artificial intelligence methods. This course offers a thorough exploration of theoretical foundations and practical applications in experimental skin regenerative medicine.
Learning Objectives- Skin Cell Culture Mastery:
Attain proficiency in cultivating and managing skin cells in 2D and 3D, emphasizing the preservation of stem cell functionality for effective regenerative applications.- Application of Cell-based and other advanced skin Regenerative approaches:
Gain insight on the use of skin regenerative therapies strategically, grounded in a solid understanding of wound healing concepts, to address specific challenges like chronic wounds, traumatic skin losses, and genetic disorders.- Advanced Gene Therapy Skills:
Development of competence in advanced skin gene therapy strategies to address inherited skin defects, encompassing both theoretical insights and practical considerations in pre-clinical models and clinical applications.- Data Analysis and AI Integration:
Explore the complexity of cellular populations in skin physiology and acquire skills in analyzing large datasets from regenerative skin approaches, incorporating artificial intelligence methods for comprehensive data management and interpretation.
Day 1. Keratinocyte Biology and culture (2D)
14.00-14:30 Reception and registration of participants
14:30-14:45 Welcome Address – Fernando Larcher; Diego Velasco
14.45-15.45 Keynote conference: Human epidermal stem cells in skin regeneration – Michele De Luca
15.45-16.15 Coffee break
16.15-16.45 Human and mouse keratinocyte culture methods I – Fernando Larcher
16.45-17.30 Human and mouse keratinocyte culture methods II. Derivation from iPSC – Christine Baldeschi
Day 2. 3D skin cultures
09.00-09.30 Overview 3D skin cultures – Fernando Larcher
09.30-10.15 Plasma-based skin equivalents – Sara Llames
10.15-10.45 Complex organotypic skin models – John Connelly
10.45-11.15 Coffee Break
11.15-11.45 Skin organoids – Julia Reichelt
11.45.12.15 Skin Bioprinting – Diego Velasco
12.15-13.30 Demonstration organotypic culture preparation – Sara Llames; Diego Velasco
13.30-14.30 Lunch Break
14.30-16.00 Demonstration Skin Bio-printing – Diego Velasco
16.00-18.00 Presentations by participants
Day 3. Wound healing and skin diseases models
09.00-09.45 Critical players in normal and impaired wound healing – Sabine Werner
09.45-10.15 GPCR in skin homeostasis and regeneration- Marta Carretero
10.15-11.00 Mesenchymal stem cells to treat chronic skin wounds – María J Escamez
11.00-11.30 Coffee break
11.30-12.15 Skin substitutes for chronic ulcers, burns and large wounds – Sara Llames
12.15-12.45 Advanced wound healing therapies – Marta Carretero
12.45-14.00 Lunch Break
14.00-14.30 Introduction 3D, KO mice, skin-humanized models – Fernando Larcher
14.30-15.15 Animal skin disease models I. Prevalent diseases – Paloma Pérez
15.15-16.00 Animal skin disease models II (rare diseases) – Alex Nystrom
16.00-16.30 Coffee break
16.30-18.00 Presentations by participants.
Day 4. Gene therapy and clinical studies
09.00-09.30 Skin gene therapy overview – Fernando Larcher
09.30-10.15 Genome editing I – Ulrich Koller
10.15-11.00 Genome editing II (in vivo) – Marta García
11.00-11.30 Coffee Break
11.30-12.15 Exon skipping / AONs – Matthias Titeux
12.15-12.45 Overview of Cell and gene therapy clinical trials for genodermatoses – Fernando Larcher, María José Escamez
12.45-13.30 Ex vivo gene therapy in the clinic – Peter Marinkovich
13.30-14.00 In vivo gene therapy in the clinic – Peter Marinkovich
14.00-15.00 Lunch Break
15.00-16.30 Demonstration: keratinocyte genome editing – TBD
16.30-18.00 Presentations by participants
20.30-22.30 Networking Dinner with participants and faculty members
Day 5. Omics and AI in dermatological research
09.00-09.45 Multiomic studies of skin biology and pathology. Overview – Carlos León
09.45-10.15 Dissecting complex cutaneous traits trough RNAseq – Ramón G. Escudero
10.15-11.00 Cell heterogeneity. Single cell RNAsec-studies – Clarisse Ganier
11.00-11.30 Coffee Break
11.30-12.00 Artificial intelligence to study rare diseases including genodermatoses – Joaquin Dopazo
12.00-14.00 Presentations by participants
14.00 Closing of the course and farewell – Fernando Larcher
Faculty
Course Chair: Fernando LARCHER, CIEMAT-UC3M, Madrid
Course Co-Chair: Diego VELASCO, UC3M, Madrid
International Speakers: Sabine WERNER, ETH, Zurich
Michele DE LUCA UNIMORE, Modena
Christine BALDESCHI iSTEM, Evry
John CONNELLY Queen Mary University, London
Peter MARINKOVICH Stanford University, USA
Ulrich KOLLER DEBRA Haus, Salzburg
Julia REICHELT Hamad Medical Corporation, Qatar
Clarisse GANIER King’s College, London
Matthias TITEUX INSERM, Paris
Alexander NYSTROM University Freiburg, Freiburg
Tutors /Local Speakers:
Sara G LLAMES CCST, Oviedo
Marta GARCÍA UC3M, Madrid
María J ESCAMEZ UC3M, Madrid
Paloma PEREZ IBV, Valencia
Marta CARRETERO CIEMAT, Madrid
Carlos LEÓN UC3M, Madrid
Ramón G ESCUDERO CIEMAT, Madrid
Joaquin DOPAZO Fundación Progreso y Saluds, Sevilla
Assistants:
Blanca DUARTE
Angélica CORRAL |
