Magyar Dermatológiai Társulat

EADV-ESDR Summer Research Workshop

Deadline for Applications: 4 February 2018

This workshop is led by Prof Caterina Missero with faculty from ESDR and is specifically aimed at dermatology residents/trainees and/or at PhD students and postdocs affiliated to dermatological research with limited experience in experimental research and in molecular biology.

The workshop will comprise a mixture of research presentations, interactive and practical sessions. A booklet of methods and protocol in advanced molecular biology will be given to participants during the course. Topics covered will include:

Gene transduction and silencing in epidermal cells:
isolation of human and mouse primary keratinocytes
keratinocyte culture and keratinocyte/fibroblast co-culture
efficient transduction of genes or gene knockdown in keratinocytes
Gene expression in skin in health and disease:
RNA isolation from human and mouse primary keratinocytes, from freshly isolated epidermis, and cells of interest from fixed skin
quantitative RT-PCR: procedure and data analysis
RNA sequencing and data analysis
Genomic editing:
designing of gene deletion or gene replacement by CRISPR/Cas9
preparation of Cas9-guide RNA vectors
assess genome editing efficiency
isolation of genome-edited cells